The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-PTP lambda antibody (ab13223)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Pancreatic carcinoma phosphatase 2
Protein-tyrosine phosphatase J
Receptor-type tyrosine-protein phosphatase U
PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.
PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
Type 1 membrane protein
PTP lambda Western Blot (ab13230)
All lanes : Anti-PTP lambda antibody (ab13223) at 1/500 dilution
Lane 1 : Human brain lysate Lane 2 : Human kidney lysate Lane 3 : Human pancreas lysate Lane 4 : Human heart lysate Lane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 5 minutes
A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).
has not yet been referenced specifically in any publications.
Publishing research using ab13230? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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