Overview

  • Product name
    Human PTP lambda peptide

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab13230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-PTP lambda antibody (ab13223)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Pancreatic carcinoma phosphatase 2
    • PCP-2
    • Protein-tyrosine phosphatase J
    • PTP-J
    • PTPRU
    • R-PTP-U
    • Receptor-type tyrosine-protein phosphatase U
    see all
  • Relevance
    PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
  • Cellular localization
    Type 1 membrane protein

Images

  • All lanes : Anti-PTP lambda antibody (ab13223) at 1/500 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human kidney lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : Human heart lysate
    Lane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
    Lane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
    Lane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
    Lane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time : 5 minutes

    A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).

References

ab13230 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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