Overview

  • Product name
    Human PTP1B ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    MCF7 extract 5 4.1%
    Inter-assay
    Sample n Mean SD CV%
    MCF7 extract 3 11.2%
  • Sample type
    Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    348 pg/ml
  • Range
    1.56 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 101 101% - 102%
    Extraction Buffer 103 100% - 107%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    PTP1B in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PTP1B protein in cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Protein tyrosine phosphatase, non-receptor type 1 (PTP1B) catalyse the hydrolysis of the phosphate monoesters specifically on tyrosine residues. PTP1B acts as a negative regulator of insulin signaling by dephosphylating the phosphotyrosine residues of insulin receptor kinase. PTP1B also dephosphoylate epidermal growth factor receptor kinase, JaK2, TYK2 kinases, PTP’s are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X PTP1B Capture Antibody 1 x 600µl
    10X PTP1B Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Plate Seals 1 unit
    PTP1B Human Lyophilized Recombinant Protein 2 vials
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • Research areas
  • Cellular localization
    Endoplasmic reticulum
  • Alternative names
    • Non receptor tyrosine phosphatase 1
    • Protein tyrosine phosphatase 1B
    • Protein tyrosine phosphatase non receptor type 1
    • Protein tyrosine phosphatase placental
    • Protein-tyrosine phosphatase 1B
    • PTP 1B
    • PTP-1B
    • PTPN 1
    • PTPN1
    • Tyrosine protein phosphatase non receptor type 1
    • Tyrosine-protein phosphatase non-receptor type 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab184865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background subtracted data from duplicate measurements are plotted.

  • The concentrations of PTP1B protein were interpolated from the PTP1B standard curve and corrected for sample dilution relative to extract load of 333 µg/mL. The mean PTP1B concentration was determined to be 42.5 ng/mL in MCF7 extract at 333 µg/mL

  • Interpolated values of PTP1B are plotted for the indicated cell lines based on an extract load of 250 µg/mL. The PTP1B detector antibody was used to analyze the same extracts by western blot (20 µg loaded/lane).

Protocols

References

ab184865 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184865.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up