Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceYDQERFDNSDLSA

Associated products

Specifications

Our Abpromise guarantee covers the use of ab45483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-PUF60 antibody (ab22819)

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Info

  • Alternative names
    • 60 kDa poly(U)-binding-splicing factor
    • FBP-interacting repressor
    • FIR
    • FLJ31379
    • FUSE-binding protein-interacting repressor
    • Poly U binding splicing factor 60KDa
    • Poly(U)-binding-splicing factor PUF60
    • poly-U binding splicing factor PUF60
    • PUF60
    • PUF60_HUMAN
    • pyrimidine tract binding splicing factor
    • Ro ribonucleoprotein-binding protein 1
    • Ro-binding protein 1
    • RoBP1
    • RoBPI
    • siah binding protein 1
    • Siah-binding protein 1
    • Siah-BP1
    • SIAHBP1
    see all
  • FunctionDNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
  • Tissue specificityIsoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the RRM half pint family.
    Contains 3 RRM (RNA recognition motif) domains.
  • DomainThe third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions.
  • Cellular localizationNucleus. Colocalizes partially with TROVE2.
  • Information by UniProt

References for Human PUF60 peptide (ab45483)

ab45483 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab45483.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"