• Product name
    Human RAGE ELISA Kit
    See all RAGE kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    EDTA plasma 5 1.3%
    Sample n Mean SD CV%
    EDTA plasma 3 7.6%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    2.4 pg/ml
  • Range
    46.87 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94.9 91.8% - 97.8%
    Cell culture media 98.6 96.8% - 101.1%
    Heparin Plasma 96.7 95.5% - 98%
    EDTA Plasma 107.2 101.7% - 116.9%
    Citrate Plasma 86.6 78% - 92.6%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Abcam’s in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of the extracellular portion of RAGE protein in Human serum, plasma and cell culture supernatant samples.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Samples diluted in Sample Diluent 50BS – 2.4 pg/mL

    Samples diluted in Sample Diluent NS – 3.2 pg/mL


  • Notes

    RAGE mediates interactions of advanced glycosylation end products (AGE). These are non-enzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. RAGE acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. RAGE interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key pro-inflammatory mediators. RAGE may be a receptor for amyloid beta peptide. RAGE contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intra-neuronal space.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)


Associated products


Our Abpromise guarantee covers the use of ab190807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • 4.5X diluted serum samples from 10 apparently healthy male donors were measured in triplicates using this kit. Interpolated data values corrected for sample dilution are graphed in pg of RAGE per mL of serum (mean +/- SD, n=3). The mean of RAGE concentration of these serum samples was determined to be 604 pg /mL with a range of 220 – 1276 pg /mL.



ab190807 has not yet been referenced specifically in any publications.

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