Overview

  • Product nameHuman Resistin ELISA Kit
    See all Resistin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 8.1%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    3.1 pg/ml
  • Range
    31 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 120 % - %
    Serum 85 % - %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Resistin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Resistin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).


    Resistin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human Resistin detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured on the plate.


    For higher sensitivity in only 90 minutes, we recommend Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
  • Tissue specificityExpressed only in fatty tissues.
  • Sequence similaritiesBelongs to the resistin/FIZZ family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Adipose tissue specific secretory factor
    • Adipose tissue-specific secretory factor
    • ADSF
    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
    • C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
    • Cysteine rich secreted protein A12 alpha like 2
    • Cysteine rich secreted protein FIZZ3
    • Cysteine-rich secreted protein A12-alpha-like 2
    • Cysteine-rich secreted protein FIZZ3
    • FIZZ 3
    • FIZZ3
    • Found in inflammatory zone 3
    • HXCP 1
    • HXCP1
    • MGC126603
    • MGC126609
    • PRO1199
    • Resistin
    • Resistin delta2
    • RETN
    • RETN 1
    • RETN_HUMAN
    • RETN1
    • UNQ407
    • XCP 1
    • XCP1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab119556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Resistin ELISA Kit images

  • Representative Standard Curve using ab119556.

Protocols

References for Human Resistin ELISA Kit (ab119556)

ab119556 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"