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|Sample type||Average %||Range|
|Cell culture supernatant||120||% - %|
|Serum||85||% - %|
Abcam’s Resistin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Resistin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Resistin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human Resistin detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured on the plate.
Get higher sensitivity in only 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-human Resistin polyclonal antibody||1 x 70µl|
|Human Resistin Standard lyophilized (4000 pg/mL upon reconstitution)||2 vials|
|Microplate coated with polyclonal antibody to Human Resistin (12 x 8 wells)||1 unit|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119556 has not yet been referenced specifically in any publications.