Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV) (ab108765)

Overview

  • Product nameHuman Anti-Respiratory syncytial virus IgG ELISA Kit (RSV)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 24 8.5%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 12 6%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Respiratory syncytial virus (RSV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Respiratory syncytial virus in Human serum and plasma.

     

    A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108765 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV) (ab108765)

    This product has been referenced in:
    • Grandin C  et al. Evidence for an intranasal immune response to human respiratory syncytial virus infection in cynomolgus macaques. J Gen Virol 96:782-92 (2015). Read more (PubMed: 25537374) »

    See 1 Publication for this product

    Product Wall

    The controls in the RSV IgG ELISA are human sera but are not available for sale apart from the kit.

    The antigens are from the Long strain. The kit has only been tested with clinical samples in which the virus strain that initiated the antibody production is unknown. The samples are expected to contain a mixture of antibodies against different RSV str...

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    Thank you for your patience in awaiting our reply. We have spoken with the laboratory and they are unsure whether this kit will react with African Green Monkey.

    Thank you for contacting us and your interest in the Respiratory Syncytial Virus (RSV) IgG Human ELISA Kit (ab108765). I am sorry for the delay in getting back to you.

    The kit is intended to be used to quantify the human anti-RSV IgG present i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"