Abcam’s anti-Respiratory syncytial virus (RSV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Anti-Respiratory syncytial virus (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Cover foil||1 unit|
|IgG Sample Diluent||yellow buffer, white cap||1 x 100ml|
|Respiratory syncytial virus anti-IgG HRP Conjugate||blue solution, black cap||1 x 20ml|
|Respiratory syncytial virus IgG Cut-off Control||yellow solution, green cap||1 x 3ml|
|Respiratory syncytial virus IgG Negative Control||yellow solution, blue cap||1 x 2ml|
|Respiratory syncytial virus IgG Positive Control||yellow solution, red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|