Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)

Overview

  • Product name
    Human Retinol binding protein 4 ELISA Kit (RBP4)
    See all Retinol binding Protein 4 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 6 ng/ml
  • Range
    6.25 ng/ml - 400 ng/ml
  • Recovery

    99.5 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Retinol binding protein 4 (RBP4) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein 4 concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.


    A Retinol binding protein 4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein 4specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein 4 captured in plate.


    Get higher sensitivity in only 90 minutes with Human Retinol Binding Protein 4 ELISA Kit (ab196264) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve Using ab108897.

Protocols

References

This product has been referenced in:
  • Al-Attar A  et al. Data correlations between gender, cytomegalovirus infection and T cells, NK cells, and soluble immune mediators in elderly humans. Data Brief 8:536-44 (2016). Read more (PubMed: 27508213) »
  • Pirazzi C  et al. PNPLA3 has retinyl-palmitate lipase activity in human hepatic stellate cells. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24670599) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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