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Abcam’s anti-Rubella virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Rubella virus in Human serum and plasma.
A 96-well plate has been precoated with Rubella virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Rubella virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rubella virus IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Rubella virus (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Rubella virus anti-IgG HRP Conjugate||Blue, Black cap||1 x 20ml|
|Rubella virus IgG Standard A – 0 U/mL||Yellow, Blue cap||1 x 2ml|
|Rubella virus IgG Standard B – 10 U/mL||Yellow, Green cap||1 x 2ml|
|Rubella virus IgG Standard C – 50 U/mL||Yellow, Yellow cap||1 x 2ml|
|Rubella virus IgG Standard D – 100 U/mL||Yellow, Red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108767 has not yet been referenced specifically in any publications.