Human SAA4 protein fragment (ab40603)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids19 to 130

Specifications

Our Abpromise guarantee covers the use of ab40603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    Western blot

    SDS-PAGE

  • Purity> 95 % by SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0.002% Sodium Azide
    Constituents: 0.1% Triton-X-100, 10mM Tris, 10mM DTT, pH 8.0

General info

  • Alternative names
    • Amyloid A, serum, 4
    • C SAA
    • C-SAA
    • Constitutively expressed serum amyloid A protein
    • CSAA
    • SAA 4
    • SAA4
    • SAA4 protein
    • SAA4_HUMAN
    • Serum amyloid A 4 protein
    • Serum amyloid A-4 protein
    • Serum amyloid A4 constitutive
    see all
  • FunctionMajor acute phase reactant. Apolipoprotein of the HDL complex.
  • Tissue specificityExpressed by the liver; secreted in plasma.
  • Sequence similaritiesBelongs to the SAA family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P35542 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Human SAA4 protein fragment images

  • Analysis of SAA4 Recombinant Protein (ab40603) @ 1µg using a 4-20% SDS gradient gel. Coomassie blue staining. A band is seen at about 17 kDa.
  • All lanes : Mouse anti-SAA4 polyclonal at 10 µg/ml

    Lane 1 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
    Lane 2 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
    Lane 3 : Recombinant A-SAA at 14 µg/ml
    Lane 4 : Human SAA4 protein fragment (ab40603) at 10 µg

    Secondary
    Goat anti-mouse IgG-HRP at 1/10000 dilution
    developed using the ECL technique

    Predicted band size : 16 kDa
    Observed band size : 16 kDa


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Human SAA4 protein fragment (ab40603)

ab40603 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot

Review text: Product was used as a positive control for C-SAA western blots of human acute phase plasma samples.
Sample: Human Tissue lysate - whole (whole human acute phase plasma)
Loading amount: 14 µg
Specification: whole human acute phase plasma
Treatment: heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
Gel Running Conditions: Reduced Denaturing (4-12% Bis-Tris)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details
Concentration: 10 µg/ml
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS, Tween-20 (0.1%)

Primary antibody (in addition to 'SAA4 protein (His tag)')
Name: Non-Abcam antibody was used: anti -SAA4, Abnova, #6291
Host species: Mouse
Clonality: Polyclonal
Dilution: 1/2000

Secondary antibody
Name: Non-Abcam antibody was used: Goat anti-mouse IgG-HRP (Dako P0447)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000

Detection
Detection method: ECL+
Exposure: 2 minute(s) and 0 second(s)
Bands: Specific: 16 kDa Non-specific: none kDa
Positive control: The product was used as the positive control in the immunoblotting experiment (visible at 16 kDa) C-SAA is also observed in human healthy control plasma and human acute phase plasma. In plasma two bands are visible for the unglycosylated (13kDa) and glycosylated (17kDa) form
Negative control: recombinant A-SAA (MBL International, #JM-4324)

Additional data
PubMed ID: 20463814: View
Additional Notes: Used PVDF membrane (Immobilon P, Millipore, #IPVH0010) and Kodak Biomax XAR film (Sigma-Aldrich, # F5513) for detection.
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Submitted Mar 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"