The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-SAE1 antibody (ab5645)
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Activator of SUMO1
Sentrin/SUMO activating protein AOS1
SUMO 1 activating enzyme E1 N subunit
SUMO 1 activating enzyme subunit 1
SUMO-activating enzyme subunit 1
Ubiquitin like protein SUMO1 activating enzyme
Ubiquitin-like 1-activating enzyme E1A
FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.