Abcam’s anti-Schistosoma mansoni IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma.
A 96-well plate has been precoated with Schistosoma mansoni antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidise (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Schistosoma mansoni-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Schistosoma mansoni IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Protein A HRP Conjugate||Blue, Black cap||1 x 20ml|
|Schistosoma mansoni (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Schistosoma mansoni IgG Cut-off Control||Yellow, Green cap||1 x 3ml|
|Schistosoma mansoni IgG Negative Control||Yellow, Blue cap||1 x 2ml|
|Schistosoma mansoni IgG Positive Control||Yellow, Red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108769 has not yet been referenced specifically in any publications.