Anti-Human Serum Albumin antibody [15C7] (ab10241)
- Datasheet
- Specific References (8)
- Protocols
Overview
- Product nameAnti-Human Serum Albumin antibody [15C7]
See all Human Serum Albumin primary antibodies - DescriptionMouse monoclonal [15C7] to Human Serum Albumin
- SpecificityNo cross-reactivity with other human proteins tested.
- Tested applicationsSuitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details
- Species reactivityReacts with: Human
Does not react with: Chicken, Cow - Immunogen
Human serum albumin.
- General notesConcentration varies from lot to lot and can be provided on request.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Purification notesPurity tested by electrophoresis.
- ClonalityMonoclonal
- Clone number15C7
- MyelomaSp2/0
- IsotypeIgG2b
- Research areas
Associated products
- Compatible Secondaries
- Related Products
- sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab10241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. | |
| ICC/IF | Use a concentration of 2.5 µg/ml. PubMed: 19497803 | |
| Sandwich ELISA | Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Human Serum Albumin (ab2406) as Detection. |
Target
- FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
- Tissue specificityPlasma.
- Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
- Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. - Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. - Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
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Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Anti-Human Serum Albumin antibody [15C7] images
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![Immunocytochemistry/ Immunofluorescence - Anti-Human Serum Albumin antibody [15C7] (ab10241)](http://a.abcam.com/ps/datasheet/images/10/ab10241/Human-Serum-Albumin-Primary-antibodies-ab10241-8.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Human Serum Albumin antibody [15C7] (ab10241)This image is courtesy of an anonymous abreview.ab10241 staining Human Serum Albumin in human embryonic stem cell derived hepatocytes by immunocytochemistry/ immunofluorescence. Cells were methanol fixed and blocked in 10% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. A FITC conjugated goat polyclonal to mouse IgG antibody, diluted 1/400, was used as the secondary (green). DAPI used to stain the nuclei (blue). -
![Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)](http://a.abcam.com/ps/products/10/ab10241/Images/ab10241-260509-AB10241.jpg)
Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)This image is courtesy of an Abreview submitted by John Andersen
Developed using the ECL technique
Predicted band size : 65 kDa
Exposure time : 5 secondsThis image is courtesy of an Abreview submitted by John Andersen
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![Sandwich ELISA - Human Serum Albumin antibody [15C7] (ab10241)](http://a.abcam.com/ps/datasheet/images/10/ab10241/Human-Serum-Albumin-Primary-antibodies-ab10241-2.jpg)
Sandwich ELISA - Human Serum Albumin antibody [15C7] (ab10241)Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) at 0.5µg/ml. -
![Western blot - Human Serum Albumin antibody [15C7] (ab10241)](http://a.abcam.com/ps/datasheet/Images/10/ab10241/ab10241_1.jpg)
Western blot - Human Serum Albumin antibody [15C7] (ab10241)
Predicted band size : 65 kDa
Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3µ g. Lane 2 - normal human serum, ~3µ g of human serum albumin. Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin.
Protocols
References for Anti-Human Serum Albumin antibody [15C7] (ab10241)
This product has been referenced in:
- Lo Giudice MC et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . Read more (PubMed: 27845346) »
- Zhu L et al. Fast fixing and comprehensive identification to help improve real-time ligands discovery based on formaldehyde crosslinking, immunoprecipitation and SDS-PAGE separation. Proteome Sci 12:6 (2014). ICC ; Human . Read more (PubMed: 24484773) »
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