Anti-Human Serum Albumin antibody [15C7] (ab10241)

Abreviews (3)Q&A (20)Specific References (14)

Overview

  • Product name
    Anti-Human Serum Albumin antibody [15C7]
    See all Human Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [15C7] to Human Serum Albumin
  • Host species
    Mouse
  • Specificity
    No cross-reactivity with other human proteins tested.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Chicken, Cow
  • Immunogen

    Human serum albumin.

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

Properties

Applications

Our Abpromise guarantee covers the use of ab10241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 2.5 µg/ml. PubMed: 19497803
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Human Serum Albumin (ab2406).

For sandwich ELISA, use this antibody as capture at 1 µg/ml with rabbit polyclonal to Human Serum Albumin (ab2406) as detection.

ab10241 can also be used as capture antibody with mouse monoclonal to Human Serum Albumin (ab37989) or HRP conjugated mouse monoclonal to Human Serum Albumin (ab24458) as a detection antibody.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • alb antibody
      • ALBU_HUMAN antibody
      • Albumin (32 AA) antibody
      • Albumin (AA 34) antibody
      • Albumin antibody
      • Analbuminemia antibody
      • Bisalbuminemia antibody
      • Cell growth inhibiting protein 42 antibody
      • DKFZp779N1935 antibody
      • Dysalbuminemic hyperthyroxinemia antibody
      • Growth inhibiting protein 20 antibody
      • HSA antibody
      • Hyperthyroxinemia dysalbuminemic antibody
      • PRO0883 antibody
      • PRO0903 antibody
      • PRO1341 antibody
      • Serum albumin antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Human Serum Albumin antibody [15C7] (ab10241)
      Immunocytochemistry/ Immunofluorescence - Anti-Human Serum Albumin antibody [15C7] (ab10241)This image is courtesy of an anonymous abreview.
      ab10241 staining Human Serum Albumin in human embryonic stem cell derived hepatocytes by immunocytochemistry/ immunofluorescence. Cells were methanol fixed and blocked in 10% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. A FITC conjugated goat polyclonal to mouse IgG antibody, diluted 1/400, was used as the secondary (green). DAPI used to stain the nuclei (blue).

      See Abreview

    • Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)
      Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)This image is courtesy of an Abreview submitted by John Andersen

      See Abreview

    • Sandwich ELISA - Anti-Human Serum Albumin antibody [15C7] (ab10241)
      Sandwich ELISA - Anti-Human Serum Albumin antibody [15C7] (ab10241)

      Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) at 0.5µg/ml.

    • Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)
      Western blot - Anti-Human Serum Albumin antibody [15C7] (ab10241)
      Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin. Detection of human serum albumin by ab10241 in western blotting. Lane 1 - human serum albumin, 3 µg. Lane 2 - normal human serum, ~3 µg of human serum albumin.

    References

    This product has been referenced in:
    • Pewkliang Y  et al. A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax. Malar J 17:50 (2018). Read more (PubMed: 29370800) »
    • Sopko R  et al. Engineering potent long-acting variants of the Wnt inhibitor DKK2. Protein Eng Des Sel 30:359-372 (2017). WB . Read more (PubMed: 28180900) »

    See all 14 Publications for this product

    Publishing research using ab10241? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    23 reviews or Q&A

    10
    Votes: Highest First





    I ordered mouse anti-human albumin antibody (15c7) ab10241.


    I want to ask you about the blocking buffer, is it ok to use BSA to prepare it?

    I prepared it by using (PBS containing 1% w/v BSA).




    According to the testing data and Abreviews from customers on the datasheet, this antibody has been used successfully with various blocking agents including BSA. For example, there is an Abeview where BSA has been used as a blocking agent in ...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Human Serum Albumin antibody [15C7]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hES derived hepatocytes)
    Specification
    hES derived hepatocytes
    Fixative
    Methanol
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2012

    what should be used for blocking for sELISA?
    Would BSA OK to ue or would Abs crossreact?

    Thank you for contacting us.

    I have heard back from the lab:
    For ab2406, for use with human tissues, BSA may work but for other species it may crossreact.
    For ab10241, it is not crossreacting with BSA.

    Thus using BSA would be ...

    Read More

    Do we have antiboduies suitable for immunoturbidimetry?
    ELISA or IP abs should work.

    Vielen Dank für Ihren Anruf.
    Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte...

    Read More

    Yes, I would like to receive a CoC for this article (with the lot number in this CoC please, it's important).

    Thank you for your reply.

    As requested, I have attached a copy of the CoC for ab10241 Human Serum Albumin antibody (15C7) including the lot number.

    I hope this will be helpful. If you have any further questions, please do not hesi...

    Read More

    Dear Madam, Sir,

    Could I have certificate of analysis of following product please:

    Produit

    Num Ref

    No de lot

    Mouse monoconal to Human Serum Albumin

    ab10241
    GR86365-1

    Plea...

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    Thank you for your enquiry.

    Some of our products are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our A...

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    I may want to test ab10241 in IHC-P.

    Thank you very much for your interest in ab10241.

    To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form ...

    Read More

    Dear technical team,

    Our customer would like to purchase human serum albumin antibody and protein was used as its immunogen.
    He is going to test IF using human sample.
    Please let me know if I can recommend ab10241 and ab67670 to th...

    Read More

    Thank you for your enquiry.

    I am sorry the immunogen used to create ab10241 Human Serum Albumin antibody [15C7] is not available. In theory,it could be used with ab67670 Human Serum Albumin protein, however you will need to consider that thi...

    Read More

    Customer would like to conjugate the abs, but needs to know if it is a kappa  or lambda chain.

    Vielen Dank für Ihren Anruf.

    Ich habe mich mit dem Labor in Verbindung gesetzt, welches diesen Antikörper herstellt. Leider wurde die leichte Kette nicht bestimmt.

    "Unfortunately we have not determined if this MAb is a kappa or ...

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    Could I have certificate of analysis of following products please:
    Product
    Référence
    N°lot
    Mouse monoconal to Human Serum Albumin
    ab10241
    3002109900
    Mouse monoclonal to Transferrin
    ab70826
    300210990...

    Read More

    Thank you for contacting us.

    Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

    Read More

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