Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455)

Overview

  • Product nameAnti-Human Serum Albumin antibody [1G12-1B3]
    See all Human Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [1G12-1B3] to Human Serum Albumin
  • Tested applicationsSuitable for: WB, IP, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Human Intestine, Vessel; HepG2 lysate; Human Serum Albumin transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab117455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Human Serum Albumin is approximately 1ng/ml as a capture antibody.

Target

  • FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Anti-Human Serum Albumin antibody [1G12-1B3] images

  • ab117455, at 5µg/ml, staining Human Serum Albumin in formalin-fixed, paraffin-embedded Human intestine, vessel tissue by Immunohistochemistry.
  • Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455) + HepG2 cell lysate

    Predicted band size : 69 kDa
  • All lanes : Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455)

    Lane 1 : Human Serum Albumin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate


    Predicted band size : 69 kDa
  • Western blot gel showing immunoprecipitation of transfected lysate using at 40 ug of ab117455 per 100 ul of Protein A Magnetic Bead.
  • Detection limit for recombinant tagged Human Serum Albumin is approximately 1ng/ml as a capture antibody.

References for Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455)

ab117455 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.

ab117455 has not yet been tested for reactivity to mouse serum albumin. It may or may not react with mouse serum albumin.

I am happy to set up the discount code for ICC with human samples. For our records...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"