Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455)

Overview

  • Product name
    Anti-Human Serum Albumin antibody [1G12-1B3]
    See all Human Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [1G12-1B3] to Human Serum Albumin
  • Tested applications
    Suitable for: WB, IP, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Human Intestine, Vessel; HepG2 lysate; Human Serum Albumin transfected 293T cell lysate
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab84348 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab117455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Human Serum Albumin is approximately 1ng/ml as a capture antibody.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Images

  • ab117455, at 5µg/ml, staining Human Serum Albumin in formalin-fixed, paraffin-embedded Human intestine, vessel tissue by Immunohistochemistry.
  • Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455) + HepG2 cell lysate

    Predicted band size : 69 kDa
  • All lanes : Anti-Human Serum Albumin antibody [1G12-1B3] (ab117455)

    Lane 1 : Human Serum Albumin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate


    Predicted band size : 69 kDa
  • Western blot gel showing immunoprecipitation of transfected lysate using at 40 ug of ab117455 per 100 ul of Protein A Magnetic Bead.
  • Detection limit for recombinant tagged Human Serum Albumin is approximately 1ng/ml as a capture antibody.

References

ab117455 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry.

ab117455 has not yet been tested for reactivity to mouse serum albumin. It may or may not react with mouse serum albumin.

I am happy to set up the discount code for ICC with human samples. For our records...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up