Overview

  • Product name
    Anti-Human Serum Albumin antibody
    See all Human Serum Albumin primary antibodies
  • Description
    Rabbit polyclonal to Human Serum Albumin
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Goat, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (LSEKERQIKK QTALVELVKH KPKATKEQLK AVMDDFAAFV EKCCKADDKE) of Human Serum Albumin (NP_000468).

  • Positive control
    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Anti-Human Serum Albumin antibody images

  • Anti-Human Serum Albumin antibody (ab83465) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Human Serum Albumin with ab83465 at 5 µg/ml.

References for Anti-Human Serum Albumin antibody (ab83465)

This product has been referenced in:
  • Wang Y  et al. Association of calreticulin expression with disease activity and organ damage in systemic lupus erythematosus patients. Exp Ther Med 13:2577-2583 (2017). Read more (PubMed: 28565882) »
  • Holzer M  et al. Refined purification strategy for reliable proteomic profiling of HDL2/3: Impact on proteomic complexity. Sci Rep 6:38533 (2016). Read more (PubMed: 27917957) »

See all 4 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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