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Human serum albumin purified from Cohn Fraction V.
Our Abpromise guarantee covers the use of ab8940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Double Immunodiffusion||Use at an assay dependent concentration. 10µL antiserum vs 5µL serum (1/20 dilution).|
|Immunoelectrophoresis||Use at an assay dependent concentration.
2µL antiserum/cm² gel vs 5µL 1/200 - 1/2000 dilution human serum IEP: 100µL antiserum vs 1µL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily be excluded.
|RID||Use at an assay dependent concentration.|
|IHC-P||1/1000. See Abreview.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab8940 at 1/1000 staining mouse embryonic tissue sections (E10.5) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with 3ml maleate bufer, 1ml FCS, 1ML 10% blocking powder before incubation with the primary antibody for 16 hours at 37°C. A Cy3® conjugated donkey anti-sheep antibody was used as the secondary.
The image shows hepatoblasts in the liver bud positive for albumin (red) and also some expressing E-cadherin (green).