Anti-Human Serum Albumin antibody [Alb1] (ab399)

Overview

  • Product nameAnti-Human Serum Albumin antibody [Alb1]
    See all Human Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [Alb1] to Human Serum Albumin
  • SpecificityAlb1 specifically recognises human albumin. < 0.8% Cross Reactivity with human AFP. Affinity constant: 3x109 l/mol human albumin.
  • Tested applicationsSuitable for: ELISA, RIA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Anti-Human Serum Albumin antibody [Alb1] images

  • Cells were grown on glass coverslips, fixed with 4% PFA/PBS for 10 minutes at room temperature and washed once with PBS. They were then treated with 250 mM Tris-HCl pH 8.0 for 10 minutes, washed twice with PBS, and permeabilized with 0.2% TritonX-100 in PBS for 20 minutes at room temperature. Cells were blocked with 5% BSA/0.1%Tween-20/PBS for 30 minutes at room temperature and incubated with ab399 in blocking buffer 1:250 for 1 hour at room temperature, then washed 3 times with PBS. They were then incubated with a goat anti-mouse-Alexa Fluor 488 (1:1000 in Blocking buffer) for 1 hour at room temperature, washed 3 times with PBS, and imaged in PBS. The Huh7.5 cells (human hepatoma, express albumin) showed a pattern of staining consistent with ER, Golgi and secretory vesicle localization. HeLa cells (do not express albumin) did not stain and BSA blocking did not cause nonspecific staining in HeLa cells.

    See Abreview

References for Anti-Human Serum Albumin antibody [Alb1] (ab399)

ab399 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Huh7.5 human hepatoma and HeLa)
Specification Huh7.5 human hepatoma and HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Constantin Takacs

Verified customer

Submitted May 15 2013

Thank you for your enquiry. All four of these antibodies are monoclonals raised to the full protein. Unfortunately the epitope recognized by each antibody is unknown, as they have not yet been epitope-mapped. All of the information we have for e...

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Sorry, we don't have the hybridoma available for sale at present.

ab399 is the only one that was not raised in rabbit but we have no data on it's use in immunohistology. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written ...

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We have no reports of it being used for this application, although with a hign affinity constant we do not see any reason it should not work for this.

Alb1 has only been tested against intact human serum albumin, so its reactivity with denatured, reduced albumin is unknown. (Biogenesis will reply regarding the other two antibodies - the reply will also be posted in the technical enquiries section o...

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This antibody has not been tested in Western blot applications

The crossreactivity of this antibody with albumin from other species is not known.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"