Full length native protein (purified) (Human).
Our Abpromise guarantee covers the use of ab399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Cells were grown on glass coverslips, fixed with 4% PFA/PBS for 10 minutes at room temperature and washed once with PBS. They were then treated with 250 mM Tris-HCl pH 8.0 for 10 minutes, washed twice with PBS, and permeabilized with 0.2% TritonX-100 in PBS for 20 minutes at room temperature. Cells were blocked with 5% BSA/0.1%Tween-20/PBS for 30 minutes at room temperature and incubated with ab399 in blocking buffer 1:250 for 1 hour at room temperature, then washed 3 times with PBS. They were then incubated with a goat anti-mouse-Alexa Fluor 488 (1:1000 in Blocking buffer) for 1 hour at room temperature, washed 3 times with PBS, and imaged in PBS. The Huh7.5 cells (human hepatoma, express albumin) showed a pattern of staining consistent with ER, Golgi and secretory vesicle localization. HeLa cells (do not express albumin) did not stain and BSA blocking did not cause nonspecific staining in HeLa cells.
ab399 has not yet been referenced specifically in any publications.