• Product nameHuman Serum Amyloid A ELISA Kit (SAA1)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.5 ng/ml
  • Range
    0.41 ng/ml - 300 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.75 86% - 102%
    Serum 114.8 105% - 124%
    Plasma 112.1 83% - 137%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Serum Amyloid A (SAA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Serum Amyloid A in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Serum Amyloid A coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum Amyloid A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Serum Amyloid A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Serum Amyloid A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.



  • Notes

    Optimization may be required with urine samples. ab100635 cross reacts with SAA2.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate



Our Abpromise guarantee covers the use of ab100635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Serum Amyloid A ELISA Kit (SAA1) images

  • Representative standard curve using ab100635


References for Human Serum Amyloid A ELISA Kit (SAA1) (ab100635)

This product has been referenced in:
  • Chang G  et al. Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver. BMC Vet Res 11:67 (2015). Read more (PubMed: 25889631) »
  • Liu J  et al. Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. PLoS One 9:e108816 (2014). Read more (PubMed: 25268271) »

See all 4 Publications for this product

Product Wall

we have not tested this kit with urine, Please note that this does not necessarily exclude the use of urine (or any other body fluid for that matter) with this kit,

Keep in mind that our ELISA kits are very accommodating and I see no reason wh...

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Thank you for your patience whilst I talked to the lab about your results. After reviewing it, there are a few points and suggestions we can give which are summarized below

You could try a 2-fold sample dilution for your serum samples (range ...

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Thank you for your inquiry. Unfortunately we are unable to sell these antibodies apart from the kit. Please let me know if I can be of further assistance.

Thank you for contacting Abcam regarding ab100635.

Regarding sample volume, please use 100ul of serum. Your sample may require further dilution, but due to variations in sample, we recommend the dilution be determined by the end user.
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Thank you for contacting us.

Our suggested dilution fold for SAA of normal human serum is 2 – 10X. Please note that the levels of the SAA may vary between different specimens. Optimal dilution factors for each sample must be deter...

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Thank you for contacting us.

Please find below therecommendations we obtained from the sources of the kits :

ab100635 : our suggested dilution fold for SAA of normal human serum is 2 – 10X. Please note that the levels of the ...

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Thank you for your inquiry.  I was in contact with the lab and they do not currently have any cross-reactivity data on feline with ab100635.  If it helps though, we do know that both the capture and detection antibodies are monoclonal and that ...

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