Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0
    • Modificationsphospho S591

Specifications

Our Abpromise guarantee covers the use of ab42772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ab42772 peptide blocks antibodies that recognise amino acids around phosphorylated serine 591 of human SHP1. This sequence is highly conserved in rat and mouse SHP1.
  • Applications

    Blocking

    ELISA

  • FormLiquid
  • Additional Notes


    Tyrosine and serine phosphorylation regulate SHP1 activity. Serine phosphorylation at Ser-591 is mediated by PKC-alpha and leads to inhibition of phosphatase activity.

    The ab42772 peptide blocks antibodies that recognise amino acids around phosphorylated serine 591 of human SHP1. This sequence is highly conserved in rat and mouse SHP1.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium Azide
    Constituents: PBS

General info

  • Alternative names
    • 70 kda SHP1L protein
    • 70Z-SHP
    • EC 3.1.3.48
    • HCP
    • HCPH
    • Hematopoietic cell phosphatase
    • Hematopoietic cell protein tyrosine phosphatase
    • Hematopoietic cell protein-tyrosine phosphatase
    • HPTP1C
    • Protein tyrosine phosphatase 1C
    • Protein tyrosine phosphatase non receptor type 6
    • Protein tyrosine phosphatase SHP1
    • Protein-tyrosine phosphatase 1C
    • Protein-tyrosine phosphatase SHP-1
    • PTN6_HUMAN
    • PTP-1C
    • PTP1C
    • PTPN6
    • SH PTP 1
    • SH-PTP1
    • SHP 1L
    • SHP1
    • SHP1L
    • Tyrosine-protein phosphatase non-receptor type 6
    see all
  • FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Target information above from: UniProt accession P29350 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Human SHP1 peptide images

  • All lanes : Anti-SHP1 (phospho S591) antibody (ab41436)

    Lane 3 : human Jurkat cells treated with pervanadate (1 mM) for 30 min
    Lane 4 : human Jurkat cells treated with pervanadate (1 mM ( for 30 min) and exposed to lambda phosphatase
    Lane 5 : human Jurkat cells treated with pervanadate (1 mM) for 30 min with phospho-SHP1 (Ser-591) peptide
    Lane 6 : human Jurkat cells treated with pervanadate (1 mM) for 30 min with non-specific phospho-serine peptide

References for Human SHP1 peptide (ab42772)

ab42772 has not yet been referenced specifically in any publications.

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