- Datasheet
- Specific References
- Protocols
Overview
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Product nameHuman SHP1 peptide
See all SHP1 proteins and peptides
Description
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NatureSynthetic
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Amino Acid Sequence
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SpeciesHuman
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Modificationsphospho Y536
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Associated products
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Corresponding Antibody
Specifications
Our Abpromise guarantee covers the use of ab42773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activityThe ab42773 peptide blocks antibodies that recognise amino acids around phosphorylated tyrosine 536 of human SHP1. The sequence is highly conserved in rat and mouse SHP1.
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Applications
Blocking - Blocking peptide for Anti-SHP1 (phospho Y536) antibody (ab41437)
ELISA
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FormLiquid
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Additional notes
SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2.
The ab42773 peptide blocks antibodies that recognise amino acids around phosphorylated tyrosine 536 of human SHP1. The sequence is highly conserved in rat and mouse SHP1. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: PBS
General Info
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Alternative names
- 70Z-SHP
- EC 3.1.3.48
- HCP
see all -
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
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Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
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Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. -
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
- Information by UniProt
Images
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Western blot - SHP1 peptide (ab42773)All lanes : Anti-SHP1 (phospho Y536) antibody (ab41437)
Lane 3 : human Jurkat cells
treated with pervanadate (1 mM) for 30 min.
Lane 4 : human Jurkat cells
treated with pervanadate (1 mM)for 30 min and exposed to alkaline
phosphatase
Lane 5 : human Jurkat cells
treated with pervanadate (1 mM) for 30 min. with phospho-SHP1 (Tyr-536)
peptide (ab42773)
References
ab42773 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"