Overview

  • Product name
    Human Siglec 9 ELISA Kit (CD329)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 130 pg/ml
  • Range
    0.123 ng/ml - 30 ng/ml
  • Recovery

    85 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.43 88% - 102%
    Serum 82.7 72% - 91%
    Plasma 78.16 70% - 91%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113339

  • Representative Standard Curve using ab113339

Protocols

References

ab113339 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately, we have not performed epitope mapping so we do not know the exact sequence where the antibody pair in the Siglec 9 Human ELISA Kit is binding. However, we do know that they are binding somewhere on ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up