General notesDiagnosis: Normal
Age: 20 years
Insoluble protein fraction.
This product belongs to a set of lysates that were extracted from a specific tissue. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below products that relate to ab44818:
Extraction 1: soluble protein fraction
ab44817 Human Skin Normal Tissue lysate
Extraction 2: insoluble protein fraction
ab44818 Human Skin Normal Tissue lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.