Human skin tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 60 years) (ab44849)

Overview

  • Product nameHuman skin tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 60 years)
    See all Skin lysates
  • Description
    Human skin insoluble fraction lysate
  • General notes

    Location: Scalp Diagnosis: Squamous cell carcinoma. Stage: Not recorded. Grade: 2 Appearance: Macroscopic Tumor is 2.5 cm in diameter, vegetative, friable, red. Microscopic Not recorded.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44849: Extraction 1: soluble protein fraction ab44843 Human skin normal tissue lysate (matched) ab44846 Human skin tumor tissue lysate Extraction 2: insoluble protein fraction ab44847 Human skin normal tissue lysate (matched) ab44849 Human skin tumor tissue lysate

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human skin tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 60 years) (ab44849)

    ab44849 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"