• NatureSynthetic

Associated products


Our Abpromise guarantee covers the use of ab16199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Blocking - Blocking peptide for Anti-SOCS3 antibody (ab16030)

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • ATOD4
    • CIS 3
    • CIS-3
    • CIS3
    • Cish3
    • Cytokine induced SH2 protein 3
    • Cytokine-inducible SH2 protein 3
    • E2a Pbx1 target gene in fibroblasts 10
    • EF 10
    • MGC71791
    • SOCS 3
    • SOCS-3
    • Socs3
    • SSI 3
    • SSI-3
    • SSI3
    • STAT induced STAT inhibitor 3
    • STAT-induced STAT inhibitor 3
    • Suppressor of cytokine signaling 3
    see all
  • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • Tissue specificityWidely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseNote=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Post-translational
    Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt

Human SOCS3 peptide images

  • All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
    Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
    Lane 8 : MOLT4 (Human) Whole Cell Lysate (ab52252) with Human SOCS3 peptide (ab16199) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Human SOCS3 peptide (ab16199)

ab16199 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot

Sample: Human Cell lysate - whole cell (Jurkat cells)
Loading amount: 10 µg
Specification: Jurkat cells
Treatment: see below (Additional notes)
Gel Running Conditions: Reduced Denaturing (5-16% gels)
Blocking step: BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Concentration: 1 µg/ml
Incubation time: 23 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)

Primary antibody (in addition to 'SOCS3 peptide')
Concentration: 1 µg/ml

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit IgG
Host species: Horse
Conjugation: Horse Radish Peroxidase
Dilution: 1/2000

Detection method: chemiluminescence
Exposure: 45 minute(s) and 0 second(s)
Bands: Specific: 25 kDa
Positive control: Jurkat cells non stimulated 30min
Jurkat cells CD3/28 stimulated 30min

Additional data
Additional Notes: Lanes:
1 = negative control / loading control (GAPDH protein)
2 = Jurkat cells non stimulated 30min
3 = Jurkat cells CD3/28 stimulated 30min
4 = SOCS 3 peptide (ab16199) 1,0µg/ml
5 = SOCS 3 peptide (ab16199) 0,5µg/ml
6 = SOCS 3 peptide (not abcam) 1µg/ml
7 = SOCS 3 peptide (not abcam) 0,5µg/ml

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Submitted Jan 06 2012