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|Sample type||Average %||Range|
|Cell culture supernatant||80.02||71% - 90%|
|Serum||80.43||72% - 91%|
|Plasma||81.36||73% - 91%|
Abcam’s Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Human Soluble TNF Receptor I||2 vials|
|Recombinant Human Soluble TNF Receptor I Standard||2 vials|
|Soluble TNF Receptor I Microplate (12 x 8 wells)||1 unit|
|Stop Solution||1 x 25ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|