Overview

  • Product name
    Human Soluble TNF Receptor I ELISA Kit
    See all TNF Receptor I kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.55 pg/ml - 400 pg/ml
  • Recovery

    80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 80.02 71% - 90%
    Serum 80.43 72% - 91%
    Plasma 81.36 73% - 91%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100642

Protocols

References

This product has been referenced in:
  • Strøm Halden TA  et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). Read more (PubMed: 24452849) »

See 1 Publication for this product

Customer reviews and Q&As

It is not known whether this kit will detect the TNF-sTNFR1 complex or not. We know it will detect free soluble TNF R1 and it does not cross react with TNF-alpha, TNF-beta or soluble TNF RII. Whether though the kit will detect soluble TNF R1 complexed ...

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Thank you for your enquiry regarding these kits.

I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample prep...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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