Human Soluble TNF Receptor II ELISA Kit (ab100643)

Overview

  • Product nameHuman Soluble TNF Receptor II ELISA Kit
    See all Soluble TNF Receptor II kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.65 91% - 112%
    Serum 104.92 95% - 115%
    Plasma 105.49 94% - 118%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Soluble TNF Receptor II ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Soluble TNF Receptor II in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Soluble TNF Receptor II coated on a 96-well plate. Standards and samples are pipetted into the wells and Soluble TNF Receptor II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Soluble TNF Receptor II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Soluble TNF Receptor II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceTNFRII (TNF Receptor II) is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF alpha but manifests a lower affinity to TNF beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNFRII consists of a 240 amino acid residue extracellular ligand binding portion, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain. Soluble TNFRII (Soluble TNF Receptor II) is a 268 amino acid isoform containing the extracellular ligand-binding portion of TNFRII. It binds to TNF alpha and is capable of inhibiting TNF alpha induced activities by acting as a decoy receptor.
  • Alternative names
    • CD120b
    • Etanercept
    • p75
    • p80 TNF alpha receptor
    • TBP2
    • TBPII
    • TNFR2
    • TNFRII
    • Tumor necrosis factor binding protein 2
    • Tumor necrosis factor receptor 2
    • Tumor necrosis factor receptor superfamily member 1B
    • Tumor necrosis factor receptor superfamily member 1b, membrane form
    • Tumor necrosis factor receptor type II
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Soluble TNF Receptor II ELISA Kit images

  • Representative standard curve using ab100643

  • Representative standard curve using ab100643

Protocols

References for Human Soluble TNF Receptor II ELISA Kit (ab100643)

ab100643 has not yet been referenced specifically in any publications.

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I can confirm that this kit (Soluble human TNF Receptor II) is suitable for detecting Etanarcept which is an alternative name for TBPII. We have not specifically tested with these drugs. As long as your samp...

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Thank you for your enquiry regarding these kits.

I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample prep...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"