The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-SPARC antibody (ab14177)
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Basement membrane protein 40
Basement-membrane protein 40
Cysteine rich protein
Secreted acidic cystein rich glycoprotein
Secreted protein acidic and cysteine rich
Secreted protein acidic and rich in cysteine
Secreted protein acidic cysteine rich
Secreted protein acidic cysteine rich (osteonectin)
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Belongs to the SPARC family. Contains 1 EF-hand domain. Contains 1 follistatin-like domain. Contains 1 Kazal-like domain.
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.