Human spleen tissue lysate - total protein (ab29699)

Overview

  • Product nameHuman spleen tissue lysate - total protein
    See all Spleen lysates
  • Description
    Human spleen total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab29699 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    Human spleen tissue lysate - total protein images

    • All lanes : Anti-Selenium Binding Protein 1 antibody (ab90135)

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Human colon tissue lysate - total protein (ab30051)
      Lane 3 : Human spleen tissue lysate - total protein (ab29699)
      Lane 4 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 20 µg per lane.


      Exposure time : 5 seconds

    References for Human spleen tissue lysate - total protein (ab29699)

    ab29699 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.






    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you.

    The credit note may be used in one of ...

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    Thank you for your reply.

    I am a little baffled as to why you are still not getting a signal, but I do not think that it is you, its probably either something really simple that we have overlooked, or its bad luck that we sent you two antibodi...

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    Thank you for your reply.

    You should be received a vial of ab67192 and a new vial of human spleen lysate tomorrow morning, hopefully this one will work for you. Your new order number is *.

    If you have any issues with this new antibody...

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    Thank you for getting back to me.

    I am really sorry about the issues that you are having. I think the best way forward is for me to send you ab67192 and a new vial of the spleen lysate (it should work as a positive control for ab67192. I would...

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    I understand your hesitation to purchase a new antibody and you could try and use 5%BSA as a blocking reagent, but the fact that you are seeing no signal what so ever makes me think that changing the block may not be successful, but it is worth a go. Read More

    Thanks for letting me know.

    I think the best way to move forward from here is to either replace the current antibody you have with ab67192:

    http://www.abcam.com/TREX1-antibody-ab67192.html



    Or, I can refund the cost ...

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    Thank you for contacting Abcam earlier today.

    I am sorry about the issues you have been having with ab83890 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples....

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this...

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    Thank you for your enquiry and your valuable feedback. We will change the shipping conditions for these products as soon as possible. This is to confirm that we will ship again one vial of each of these lysates as free of charge replacements - on d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"