Human Stem Cell Factor ELISA Kit (Kit Ligand) (ab108901)

Overview

  • Product nameHuman Stem Cell Factor ELISA Kit (Kit Ligand)
    See all Stem Cell Factor kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.1 ng/ml
  • Range
    0.156 ng/ml - 40 ng/ml
  • Recovery

    95 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Stem Cell Factor (Kit Ligand) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Stem Cell Factor concentrations in Human plasma, serum and cell culture supernatants.

    A Stem Cell Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Stem Cell Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Stem Cell Factor captured in plate.

     

    Store kit at 4°C immediately upon receipt, apart from the Streptavidin-Peroxidase Conjugate & Biotinylated Antibody, which should be stored at -20°C.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Stem Cell Factor ELISA Kit (Kit Ligand) images

  • Representative Standard Curve using ab108901.

Protocols

References for Human Stem Cell Factor ELISA Kit (Kit Ligand) (ab108901)

ab108901 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108901.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"