Human stomach tissue (infiltrating carcinoma) - soluble fraction (male, 42 years) (differentiated) (ab44284)

Overview

  • Product nameHuman stomach tissue (infiltrating carcinoma) - soluble fraction (male, 42 years) (differentiated)
    See all Stomach lysates
  • Description
    Human stomach soluble fraction lysate
  • General notesTissue: Stomach Location: Lesser curvature Diagnosis: Infiltrating carcinoma, well differentiated. Stage: n/a Grade: n/a Sex: Male Age: 42 years Gross findings: Hard thick ulceration at the lesser curvature, measures 3cm by 3.5cm in diameter. Dark brown in colour. Cut section is hard and white. Base is firm, gritty and hemorrhagic. Microscopic findings: Biopsy shows malignant columnar cells arranged in glands, invading the muscle. There is evidence of mucinous secretion. Nuclei are irregular with large nucleoli. Many monstrous nuclei. The malignant cells have invaded the gastric wall. Soluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44284: Extraction 1: soluble protein fraction ab44284 Human Stomach Tumor Tissue lysate ab44283 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44286 Human Stomach Tumor Tissue lysate ab44285 Human Stomach Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 0.25% Sodium Deoxycholate, 0.1% SDS, PBS, 1µg/ml Pepstatin A, 1µg/ml Leupeptin, 1µg/ml Aprotinin, 1mM PMSF, 1mM EDTA, 1mM Sodium fluoride, 1mM Sodium Orthovanadate, pH 7.4
    Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human stomach tissue (infiltrating carcinoma) - soluble fraction (male, 42 years) (differentiated) (ab44284)

    ab44284 has not yet been referenced specifically in any publications.

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