Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 54 years) (ab44299)


  • Product nameHuman stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 54 years)
    See all Stomach lysates
  • Description
    Hu stomach insoluble fraction lysate
  • General notesTissue: Stomach Location: Lesser curvature Diagnosis: Adenocarcinoma, poorly differentiated Stage: IIIA T3N1M0 Grade: n/a Sex: Male Age: 54 years Gross findings: Ulcerative lesion, ill demarcated. Tumor measures 4cm x 5cm x 5cm in diameter. Cut section is firm and white. Paragastric lymph nodes: 4. Microscopic findings: Tissue sections show proliferation of malignant cells. The epithelial cells have small, basophilic irregular nuclei with abundant cytoplasm. Cytoplasm contains much mucin. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are not evident. The neoplastic cells invade the smooth muscle proporia. The stroma is infiltrated by small numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Blood vessel invasions are not revealed. Lymph nodes show metastases. Insoluble protein fraction

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44299: Extraction 1: soluble protein fraction ab44297 Human Stomach Tumor Tissue lysate ab44296 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44299 Human Stomach Tumor Tissue lysate ab44298 Human Stomach Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 54 years) (ab44299)

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