Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 66 years) (ab44466)


  • Product nameHuman stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 66 years)
    See all Stomach lysates
  • Description
    Human stomach insoluble fraction
  • General notes

    Tissue: Stomach Location: Pylorus. Diagnosis: Adenocarcinoma. Well differentiated. Stage: II Grade: n/a Sex: Male Age: 66 years Gross findings: Ulcerated and vegetated lesion, located at the pylorus. Tumor measures 5 cm x 5 cm in diameter. Gray in color, wall 1.5 cm thick, firm, dirty, and hemorrhagic. Microscopic findings: Biopsy shows malignant columnar cells arranged in glands. The malignant cells have clear cytoplasm with vacuoles. Nuclei have irregular nuclear membranes, cytoplasm is granular, or vacuolated with large nucleoli. Many monstrous nuclei. The malignant cells have invaded the gastric wall. Many inflammatory cells.


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44466: Extraction 1: soluble protein fraction ab44464 Human Stomach Tumor Tissue lysate ab44463 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44466 Human Stomach Tumor Tissue lysate ab44465 Human Stomach Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

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