Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 72 years) (differentiated) (ab44251)

Overview

  • Product nameHuman stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 72 years) (differentiated)
    See all Stomach lysates
  • Description
    Human stomach insoluble fraction
  • General notes

    Tissue: Stomach Location: Antrum. Diagnosis: Adenocarcinoma. Stage: III T2N0Mx. Grade: 2 Sex: Male Age: 72 years Gross findings: Tumor size 3 cm., moderately differentiated. Cut section friable, homogeneous, and pink/red. Hemorrhagic and invasive. Insoluble protein fraction.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44251: Extraction 1: soluble protein fraction ab44249 Human stomach tumor tissue lysate ab44248 Human stomach normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab44251 Human stomach tumor tissue lysate ab44250 Human stomach normal tissue lysate (matched).

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 72 years) (differentiated) (ab44251)

    ab44251 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"