Human stomach tissue lysate (adenocarcinoma) - soluble fraction (female, 46 years) (ab44383)

Overview

  • Product nameHuman stomach tissue lysate (adenocarcinoma) - soluble fraction (female, 46 years)
    See all Stomach lysates
  • Description
    Human stomach soluble fraction lysate
  • General notes

    Location: Antrum Diagnosis: Adenocarcinoma, moderately differentiated Stage: III T3N1M0 Grade: n/a Gross findings: Ulcerative lesion, ill demarcated. Tumor size 4cm in diameter. Cut section firm and white. Paragastric lymph nodes: 7. Microscopic findings: Tissue sections show proliferation of malignant cells. Mitotic figures are not evident. The epithelial cells have small irregular basophilic nuclei and abundant cytoplasm. Cytoplasm contains much mucin. Nuclear chromatin is coarse with prominent nuclei. The neoplastic cells form glands invading smooth muscle proporia and serosa. The stroma is infiltrated by small numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Blood vessel invasions are not revealed. The lymph nodes show metastases.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44383: Extraction 1: soluble protein fraction ab44383 Human Stomach Tumor Tissue lysate ab44382 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44385 Human Stomach Tumor Tissue lysate ab44384 Human Stomach Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human stomach tissue lysate (adenocarcinoma) - soluble fraction (female, 46 years) (ab44383)

    ab44383 has not yet been referenced specifically in any publications.

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