Human stomach tissue lysate (infiltrating carcinoma) - insoluble fraction (male, 75 years) (ab44291)


  • Product nameHuman stomach tissue lysate (infiltrating carcinoma) - insoluble fraction (male, 75 years)
    See all Stomach lysates
  • Description
    Hu stomach insoluble fraction lysate
  • General notesTissue: Stomach Location: Left upper quadrant Diagnosis: Infiltrating carcinoma, poorly differentiated. Stage: n/a Grade: n/a Sex: Male Age: 75 years Gross findings: Ulcerated, vegetated tumor, measures 3cm by 3cm in diameter. Base is firm, dirty and hemorrhagic. Wall is firm. Microscopic findings: Biopsy shows malignant columnar cells arranged in glands. The malignant cells have clear cytoplasm with vacuoles. There is evidence of mucinous secretion. Nuclei are irregular with large nucleoli. Many monstrous nuclei and several mitoses. The malignant cells have invaded the gastric wall. Insoluble protein fraction

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44291: Extraction 1: soluble protein fraction ab44288 Human Stomach Tumor Tissue lysate ab44287 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44291 Human Stomach Tumor Tissue lysate ab44290 Human Stomach Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
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