The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-STUB1 antibody (ab2917)
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human CHIP, catalog ab2917. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Antigen NY CO 7
C terminus of Hsp70-interacting protein
Carboxy terminus of Hsp70 interacting protein
Carboxy terminus of Hsp70-interacting protein
Carboxy terminus of Hsp70p interacting protein
CLL associated antigen KW 8
CLL-associated antigen KW-8
E3 ubiquitin protein ligase CHIP
E3 ubiquitin-protein ligase CHIP
Heat shock protein A binding protein 2 (c terminal)
NY CO 7
Serologically defined colon cancer antigen 7
STIP1 homology and U Box containing protein 1
STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase
STIP1 homology and U box-containing protein 1
FunctionE3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
Tissue specificityHighly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
PathwayProtein modification; protein ubiquitination.