Overview

  • Product name
    Human Survivin ELISA Kit
    See all Survivin kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Survivin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Survivin in cell culture supernatants, serum and plasma (heparin, EDTA).


    A Survivin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Survivin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Survivin amount of sample captured in plate.


    Get better reproducibility in only 90 minutes with Human Survivin ELISA Kit (ab183361) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab119607

Protocols

References

ab119607 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Vielen Dank für Ihre Anfrage.

Frau Burkhardt ist nun in Ihrem wohlverdienten Urlaub, daher werde ich mich um Ihren Fall kümmern.

Der Schritt, welcher entfällt, ist der folgende:

"7. Preparation "A...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

Read More

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up