Human Survivin ELISA Kit (ab183361)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameHuman Survivin ELISA Kit
See all Survivin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Jurkat cells 5 2.5% Inter-assay Sample n Mean SD CV% Jurkat cells 3 5.5% -
Sample typeCell culture supernatant, Urine, Serum, Adherent cells, Suspension cells, Tissue Extracts, Citrate Plasma
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Assay typeSandwich (quantitative)
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Sensitivity7 pg/ml
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Range93.75 pg/ml - 6000 pg/ml
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Recovery
79.5 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 86.4 85.4% - 87.4% Urine 91.6 89.9% - 92.6% Serum 79.5 78.5% - 80.5% Citrate Plasma 99.2 94.6% - 102.3% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Human
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Product overview
Survivin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit (ab183361) is designed for the quantitative measurement of Survivin protein in human cell culture supernatant, serum, plasma, urine, cell and tissue extract samples.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS - 28 pg/mL
Samples diluted in Sample Diluent 75BP - 7 pg/mL
Samples diluted in 1X Cell Extraction Buffer - 25 pg/mL -
Notes
Survivin is a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and it participates in the organization of the center spindle by associating with polymerized microtubules. Survivin complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Survivin may counteract a default induction of apoptosis in G2/M phase. The acetylated form of Survivin represses STAT3 transactivation of target gene promoters. Survivin may play a role in neoplasia. Survivin is an inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 of Survivin do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Survivin Capture Antibody 1 x 600µl 10X Survivin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml ab221825 - Antibody Diluent 5BI 1 x 6ml Plate Seals 1 unit Sample Diluent 75BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 96 tests Stop Solution 1 x 12ml Survivin Human Lyophilized Recombinant Protein 2 vials TMB Substrate 1 x 12ml -
Research areas
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FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
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Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
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Sequence similaritiesBelongs to the IAP family.
Contains 1 BIR repeat. -
Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
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DomainThe BIR repeat is necessary and sufficient for HBXIP binding.
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Post-translational
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
- Information by UniProt
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Alternative names
- API4
- Apoptosis inhibitor 4
- Apoptosis inhibitor survivin
see all -
Database links
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: O15392 Human
- Unigene: 514527 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab183361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Human survivin standard curve comparison data.Standard curve comparison between human survivin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of Survivin standard curve for cell culture supernatant and urine samples measurementsFigure 1. Example of Survivin standard curve for cell culture supernatant and urine samples measurements. Background-subtracted data values (mean +/- SD) are graphed.
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Example of Survivin standard curve for serum and plasma-citrate samples measurements.Figure 2. Example of Survivin standard curve for serum and plasma-citrate samples measurements. Background-subtracted data values (mean +/- SD) are graphed.
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Example of Survivin standard curve for cell and tissue extract samples measurements.Figure 3. Example of Survivin standard curve for cell and tissue extract samples measurements. Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Jurkat cell extract within the working range of the assay.Figure 4. Titration of Jurkat cell extract within the working range of the assay. Background-subtracted data values (mean +/- SD, n=2) are graphed.
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Survivin concentrations in 10 individual Human serum donors.Figure 5. Survivin concentrations in 10 individual Human serum donors. Two fold diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in pg of Survivin per mL of serum (mean +/- SD, n=3).
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Comparison of Survivin concentrations in media and MCF7 cell supernatants.Figure 6. Comparison of Survivin concentrations in media and MCF7 cell supernatants. MCF7 cells were grown in 10F HGDMEM medium and Survivin concentrations were measured in undiluted cell culture supernatant (SN) sample and the 10F HGDMEM medium using this kit. Interpolated data values are graphed (mean +/- SD, n=2). Note that no detectable Survivin concentrations in growth medium were observed.
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Comparison of Survivin concentrations in control and nocodazole treated HeLa cells.Figure 7. Comparison of Survivin concentrations in control and nocodazole treated HeLa cells. HeLa cells were grown in 10F HGDMEM medium for 17 hours in the presence of 200 ng/mL nocodazole or drug’s vehicle (DMSO). Survivin concentrations were measured in cell extracts diluted to 125 and 62.5 µg/mL using this kit. Interpolated data values expressed in pg Survivin per g of cell extract are graphed (mean +/- SD, n=2).
Protocols
References
This product has been referenced in:
- Liu Y et al. Identification of bile survivin and carbohydrate antigen 199 in distinguishing cholangiocarcinoma from benign obstructive jaundice. Biomark Med 11:11-18 (2017). Sandwich ELISA ; Human . Read more (PubMed: 27874286) »
Customer reviews and Q&As
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