Overview

  • Product name
    Human Syndecan-1 ELISA Kit (CD138)
    See all Syndecan-1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 10.2%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 4.94 ng/ml
  • Range
    8 ng/ml - 256 ng/ml
  • Recovery

    101 %

  • Assay time
    1h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Syndecan-1 (CD138) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.

    This kit will recognize both endogenous and recombinant Human Syndecan-1.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
  • Sequence similarities
    Belongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • Cellular localization
    Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • Alternative names
    • CD138
    • CD138 antigen
    • SDC
    • Sdc1
    • SDC1_HUMAN
    • SYND1
    • Syndecan 1
    • Syndecan-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Syndecan-1 ELISA Kit (CD138) images

  • Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).

  • Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).

  • Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).

  •  Representative Standard Curve using ab46506

Protocols

References for Human Syndecan-1 ELISA Kit (CD138) (ab46506)

This product has been referenced in:
  • Puerta-Guardo H  et al. Dengue Virus NS1 Disrupts the Endothelial Glycocalyx, Leading to Hyperpermeability. PLoS Pathog 12:e1005738 (2016). Read more (PubMed: 27416066) »
  • Shimizu Y  et al. A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med 14:31 (2016). ELISA ; Human . Read more (PubMed: 26830497) »

See all 4 Publications for this product

Product Wall

Thank you for your call earlier this week and for your patience while I've been in touch with the lab regarding your enquiry.

The control provided in the kit is the same protein as the standard, and they are both native purified protein isola...

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Thank you for contacting us.

In order to measure syndecan-1 from cell culture supernatants, spinning at 1000g during 10 minutes is necessary to remove particulates and aggregates.

How was the thaw process performed? Was it by heatin...

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ab46506, CD138 ELISA kit Antibodies are raised in mouse.

I hope this helps. Please contact me again if you have any further questions.

Thank you for your enquiry. The standard used is natural protein coming from U266 cells supernatent (unpurified).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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