Overview

  • Product nameHuman Syndecan-1 ELISA Set (without plates)
    See all Syndecan-1 kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2.56 ng/ml
  • Range
    8 ng/ml - 256 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Syndecan-1 Human in vitro ELISA Set is designed for the quantitative measurement of Syndecan-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human Syndecan-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    The Syndecan-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human Syndecan-1.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

  • FunctionCell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
  • Sequence similaritiesBelongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • Cellular localizationMembrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • Alternative names
    • CD138
    • CD138 antigen
    • SDC
    • Sdc1
    • SDC1_HUMAN
    • SYND1
    • Syndecan 1
    • Syndecan-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human Syndecan-1 ELISA Set (without plates) images

    • Syndecans standard curve ranging from 8 to 256 ng/ml

    References for Human Syndecan-1 ELISA Set (without plates) (ab47352)

    ab47352 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab47352.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"