Overview

  • Product nameHuman TARC ELISA Kit
    See all TARC kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.7 98% - 122%
    Serum 76.75 70% - 93%
    Plasma 78.32 73% - 97%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TARC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TARC in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human TARC coated on a 96-well plate. Standards and samples are pipetted into the wells and TARC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TARC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TARC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionChemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
  • Tissue specificityExpressed at high levels in thymus and at low levels in the lung, colon and small intestine.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • A 152E5.3
    • ABCD 2
    • C C motif chemokine 17
    • C-C motif chemokine 17
    • CC chemokine TARC
    • CCL 17
    • CCL17
    • CCL17_HUMAN
    • Chemokine (C C motif) ligand 17
    • Chemokine ligand 17
    • MGC138271
    • MGC138273
    • SCYA17
    • Small inducible cytokine A17
    • Small inducible cytokine subfamily A (Cys Cys) member 17
    • Small inducible cytokine subfamily A member 17
    • Small-inducible cytokine A17
    • T cell directed CC chemokine
    • Thymus and activation regulated chemokine
    • Thymus and activation-regulated chemokine
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human TARC ELISA Kit images

  • Representative standard curve using ab100644

Protocols

References for Human TARC ELISA Kit (ab100644)

ab100644 has not yet been referenced specifically in any publications.

Product Wall

CCL17 secretion by human macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL17 secretion by human macrophages isolated by negative selection in culture for 3 and 7 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by higher secretion by M2a macrophages (MSCF+IL4) on day 7 compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethason IL10=MCSF+IL10 TGFb=MCSF+TGFb)
Username

Abcam user community

Verified customer

Submitted Apr 01 2014

Thanks for your inquiry. Based upon sequence homology, I would not expect any of the antibodies to cross react with mouse as there is only a 64% homology. Please let me know if you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"