For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam’s Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|10X Wash Solution||1 x 50ml|
|Anti-Testosterone IgG Coated Microplate (12 x 8 wells)||1 unit|
|Cover Foil||1 unit|
|Free Testosterone Control A||1 x 1ml|
|Free Testosterone Control B||1 x 1ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|Testosterone HRP Conjugate||1 x 15ml|
|Testosterone Standard 0 - 0 pg/mL||1 x 1ml|
|Testosterone Standard 1 - 0.2 pg/mL||1 x 1ml|
|Testosterone Standard 2 - 1.0 pg/mL||1 x 1ml|
|Testosterone Standard 3 - 4.0 pg/mL||1 x 1ml|
|Testosterone Standard 4 - 20 pg/mL||1 x 1ml|
|Testosterone Standard 5 - 100 pg/mL||1 x 1ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab178663 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"