Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | < 10% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | < 12% |
96
%
Sample type | Average % | Range |
---|---|---|
Cell culture supernatant | 97.87 | 85% - 104% |
Serum | 94.46 | 82% - 102% |
Plasma | 95.78 | 93% - 103% |
Human TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100647) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Components | 1 x 96 tests |
---|---|
20X Wash Buffer Concentrate | 1 x 25ml |
500X HRP-Streptavidin Concentrate | 1 x 200µl |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-Human TGF beta 1 (lyophilized) | 2 vials |
Recombinant Human TGF beta 1 Standard | 2 vials |
Stop Solution | 1 x 8ml |
TGF beta 1 Microplate (12 x 8 wells) | 1 unit |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"