Human TGF beta 1 ELISA Kit (ab100647)
- Datasheet
- Specific References (3)
- Protocols
Overview
- Product nameHuman TGF beta 1 ELISA Kit
See all TGF beta 1 kits - Detection methodColorimetric
- Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% - Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 80 pg/ml
- Range0.082 ng/ml - 60 ng/ml
- Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.87 85% - 104% Serum 94.46 82% - 102% Plasma 95.78 93% - 103% - Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s TGF beta 1 (TGFB1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human TGF beta 1 in serum, plasma, cell culture supernatants.
Many cells synthesize TGFβ1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
This assay employs an antibody specific for human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Optimization may be required with urine samples.
- Tested applicationsSuitable for: Sandwich ELISAmore details
- PlatformMicroplate
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human TGF beta 1 2 vials Recombinant Human TGF beta 1 Standard 2 vials Stop Solution 1 x 8ml TGF beta 1 Microplate (12 x 8 wells) 1 x 1µl TMB One-Step Substrate Reagent 1 x 12ml - Research areas
- FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
- Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
- Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
- Sequence similaritiesBelongs to the TGF-beta family.
- Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. - Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Alternative names
- Camurati-Engelmann disease
- CED
- DPD1
see all -
Database links
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
Applications
Our Abpromise guarantee covers the use of ab100647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Human TGF beta 1 ELISA Kit images
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Sandwich ELISA - TGF beta1 Human ELISA Kit (ab100647)Standard curve in Assay Diluent A : mean of duplicates (+/-SD)
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Sandwich ELISA - TGF beta1 Human ELISA Kit (ab100647)Standard curve in Assay Diluent B : mean of duplicates (+/-SD)
Protocols
References for Human TGF beta 1 ELISA Kit (ab100647)
This product has been referenced in:
- Recha-Sancho L & Semino CE Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering. PLoS One 11:e0157603 (2016). Read more (PubMed: 27315119) »
- Fernández-Muiños T et al. Bimolecular based heparin and self-assembling hydrogel for tissue engineering applications. Acta Biomater 16:35-48 (2015). Read more (PubMed: 25595471) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"