• Product name
    Human TGF beta 1 ELISA Kit
    See all TGF beta 1 kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    0.082 ng/ml - 60 ng/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.87 85% - 104%
    Serum 94.46 82% - 102%
    Plasma 95.78 93% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100647) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TGF beta 1 (lyophilized) 2 vials
    Recombinant Human TGF beta 1 Standard 2 vials
    Stop Solution 1 x 8ml
    TGF beta 1 Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Standard curve in Assay Diluent A : mean of duplicates (+/-SD)

  • Standard curve in Assay Diluent B : mean of duplicates (+/-SD)



This product has been referenced in:
  • Recha-Sancho L & Semino CE Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering. PLoS One 11:e0157603 (2016). Read more (PubMed: 27315119) »
  • Tian Y  et al. Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-ß signaling. Cancer Sci 107:274-80 (2016). Read more (PubMed: 26749136) »

See all 5 Publications for this product

Customer reviews and Q&As

TGFb human macrophage and DC secretion

Excellent Excellent 5/5 (Ease of Use)
TGFb human macrophage & DC secretion in culture for 3, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by TGFb secretion by different macrophages subtyes & DC.

Abcam user community

Verified customer

Submitted May 02 2014

human TGF beta 1 detection in M6 cells

Excellent Good 4/5 (Ease of Use)
The Kit works without any remarkable problem.
It isn't a very consuming time protocol.
As control we use mouse cells supernatant and the result was very low.

Abcam user community

Verified customer

Submitted Oct 23 2013

Thank you for contacting us. Yes, you can store the HCl and the Urea/Acetic acid for later use, you do not have to use the diluted versions all at once. Please let me know if you have any further questions.

Avec ab100647, nous recommandons d’activer vos échantillons en faisant une extraction à l'acide, voir page 9 -10 du protocole.

Il est fortement recommandé d’effectuer cette l'extraction à l'a...

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Both antibodies are produced in Rat and monoclonal.

The laboratory that developed the ELISA did not send data showing results +/- acid treatment but did send the following summary. Please let me know if you still have questions or concerns.

It is strongly recommended that the customer perform...

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Thank you for contacting Abcam regarding ab100647.

I have received some further information from the lab regarding this issue and I wanted to share this with you.

The appearance of Assay Diluent B can vary by batch but a little cl...

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Thank you again for your call yesterday, and I just want to follow-up to make sure your experimentwent smoothly.

Please keep me updated about the results using the modified diluent, and let me know if you have any questions or if there is any...

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Thank you for calling Abcam earlier today.
I have talked to the lab and they have told me that Assay Diluent B does contain some protein and solutes so it would not be uncommon to see a little cloudiness or floating unsolubilized protein aggregate...

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Thank yhou for your enquiry.

I have copied some information belowproviding tips for sample preparation when using this kit, which includes human serum prepration.

I hope this will be helpful to you. if you have any further questio...

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1-10 of 15 Abreviews or Q&A


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