Overview

  • Product name
    Human TGF beta 1 ELISA Kit
    See all TGF beta 1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    0.082 ng/ml - 60 ng/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.87 85% - 104%
    Serum 94.46 82% - 102%
    Plasma 95.78 93% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100647) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Ab100647 was reformulated on 31st May 2018 with new capture and detector antibodies that allows to increase the sensitivity to human TGF beta 1.


    Please note that as a consequence of this change, the procedure and protocol have slightly changed and we encourage you to review the protocol steps before starting your experiments

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human TGF beta 1 (lyophilized) 2 vials
    Recombinant Human TGF beta 1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TGF beta 1 Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab100647 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Protocols

    References

    This product has been referenced in:
    • Corcelli M  et al. Neuroprotection of the hypoxic-ischemic mouse brain by human CD117+CD90+CD105+amniotic fluid stem cells. Sci Rep 8:2425 (2018). Read more (PubMed: 29402914) »
    • Wang X  et al. Cancer-associated fibroblasts induce epithelial-mesenchymal transition through secreted cytokines in endometrial cancer cells. Oncol Lett 15:5694-5702 (2018). Read more (PubMed: 29563996) »

    See all 7 Publications for this product

    Publishing research using ab100647? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    15 reviews or Q&A

    10
    Votes: Highest First

    TGFb human macrophage and DC secretion

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    TGFb human macrophage & DC secretion in culture for 3, isolated by negative selection.
    The assay is easy to use and takes about 5.30hr.
    Good specificity as shown by TGFb secretion by different macrophages subtyes & DC.
    M0 = MCSF M1 = MCSF+IFNg M2 = MCSF+IL4 DC = GMCSF+IL4.
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    Abcam user community

    Verified customer

    Submitted May 02 2014

    human TGF beta 1 detection in M6 cells

     Excellent Good 4/5 (Ease of Use)
    Abreviews
    The Kit works without any remarkable problem.
    It isn't a very consuming time protocol.
    As control we use mouse cells supernatant and the result was very low.
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    Username

    Abcam user community

    Verified customer

    Submitted Oct 23 2013

    can i store extra reagents until i use next?

    Thank you for contacting us. Yes, you can store the HCl and the Urea/Acetic acid for later use, you do not have to use the diluted versions all at once. Please let me know if you have any further questions.

    Pouvez-vous me dire si votre kit est suffisamment sensible pour être utilisé sur des échantillons non activés car nous souhaiterions uniquement détecter la TGFb actif naturellement sécrétée par les...

    Read More



    Avec ab100647, nous recommandons d’activer vos échantillons en faisant une extraction à l'acide, voir page 9 -10 du protocole.

    Il est fortement recommandé d’effectuer cette l'extraction à l'a...

    Read More

    I would like to know the hosts used to produce the capture and detection antibodies used in this kit? Also I'd like to know the clonality (poly or mono) of these antibodies.

    Both antibodies are produced in Rat and monoclonal.

    For the TGF beta ELISA assay, what is the rationale for treating cell culture supernatants with 1 N HCl before assaying? The protocol states that it is to "To activate latent TGF beta 1 to the immunoreactive form". Does that mean the epitopes...

    Read More

    The laboratory that developed the ELISA did not send data showing results +/- acid treatment but did send the following summary. Please let me know if you still have questions or concerns.

    It is strongly recommended that the customer perform...

    Read More

    The Assay Diluent B 5x was cloudy upon arrival. Is this normal or is the sample contaminated?

    Thank you for contacting Abcam regarding ab100647.

    I have received some further information from the lab regarding this issue and I wanted to share this with you.

    The appearance of Assay Diluent B can vary by batch but a little cl...

    Read More

    Inquiry: Assay Diluent B contaminated with bacteria. Can you give me a call? Emergency!!! I am in the middle of the experiment.

    Thank you again for your call yesterday, and I just want to follow-up to make sure your experimentwent smoothly.

    Please keep me updated about the results using the modified diluent, and let me know if you have any questions or if there is any...

    Read More

    The Assay Diluent B 5x was cloudy upon arrival. Is this normal or is the sample contaminated?

    Thank you for calling Abcam earlier today.
    I have talked to the lab and they have told me that Assay Diluent B does contain some protein and solutes so it would not be uncommon to see a little cloudiness or floating unsolubilized protein aggregate...

    Read More

    Dear Sir or Madam,
    I would be interested in the TGF beta 1 Human ELISA Kit - 1 x 96 Well Plate (ab100647). I will have to measure tgf concentration in human serum samples. Could you please provide information about the serum preparation specific f...

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    Thank yhou for your enquiry.

    I have copied some information belowproviding tips for sample preparation when using this kit, which includes human serum prepration.

    I hope this will be helpful to you. if you have any further questio...

    Read More

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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