• Product nameHuman TGF beta 1 ELISA Kit
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 3.1%
    Sample n Mean SD CV%
    Overall 7.6%
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.03 ng/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TGF beta 1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TGF beta 1 concentrations in cell culture supernatants.

    A TGF beta 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TGF beta 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TGF beta 1 captured in plate. This assay recognizes both natural and recombinant Human TGF beta 1.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseCamurati-Engelmann disease
  • Sequence similaritiesBelongs to the TGF-beta family.
  • DomainThe 'straitjacket' and 'arm' domains encircle the growth factor monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. Activation of TGF-beta1 requires the binding of integrin alpha-V to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-beta binding proteins. The sheer physical force unfastens the straitjacket and releases the active growth factor dimer.
  • Post-translational
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • LAP
    • Latency-associated peptide
    • TGF-beta-1
    • tgfb1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human TGF beta 1 ELISA Kit images

  • Representative Standard Curve using ab108912.


References for Human TGF beta 1 ELISA Kit (ab108912)

ab108912 has not yet been referenced specifically in any publications.

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