Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (ab108907)

Overview

  • Product nameHuman Thrombin-Antithrombin Complex ELISA Kit (TAT)
    See all Thrombin-Antithrombin Complex kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 11.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1 ng/ml
  • Range
    1.481 ng/ml - 120 ng/ml
  • Recovery

    95 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.

    A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Cellular localizationSecreted
  • Alternative names
    • antithrombin III
    • AT3
    • AT3D
    • ATIII
    • coagulation factor II (thrombin)
    • F2
    • PRO0309
    • prothrombin
    • prothrombin B chain
    • PT
    • RPRGL2
    • serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
    • serine protease
    • serine-cysteine proteinase inhibitor clade C member 1
    • serpin C1
    • serpin peptidase inhibitor, clade C (antithrombin), member 1
    • SERPINC1
    • TAT
    • THPH1
    • THPH7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Thrombin-Antithrombin Complex ELISA Kit (TAT) images

  • TAT measured in biological fluids, with background signal subtracted (duplicates +/- SD).

  • Representative Standard Curve Using ab108907.

Protocols

References for Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (ab108907)

This product has been referenced in:
  • Wang J  et al. Prothrombotic SERPINC1 gene polymorphism may affect heparin sensitivity among different ethnicities of Chinese patients receiving heart surgery. Clin Appl Thromb Hemost 21:760-7 (2015). Read more (PubMed: 25361738) »
  • Hyacinth HI  et al. Effect of Chronic Blood Transfusion on Biomarkers of Coagulation Activation and Thrombin Generation in Sickle Cell Patients at Risk for Stroke. PLoS One 10:e0134193 (2015). Read more (PubMed: 26305570) »

See all 3 Publications for this product

Product Wall

Abreviews
Human Thrombin-Antithrombin Complex ELISA Kit Abcam Review:

An experienced laboratory technician completed this Thrombin-Antithrombin ELISA from Abcam, catalog number AB108907, Lot GR256365-9. The goal of the experiment was to analyze the linearity of dilutions of 3 different rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross-reactivity for rhesus macaque.

Overall, the kit, like other Abcam ELISA kits, was easy to follow, and was straight-forward in directions, while also being brief. The laboratory technician had no problem understanding any parts of the protocol.

Our experiment consisted of using EDTA-prepared rhesus macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted 1:5, 1:10, 1:20, and 1:40 in the kit’s diluent, and tested in duplicate, as were the standards. The rest of the assay was performed to protocol’s specifications.

The kit recommended 5 standard dilutions. We noted that the highest concentration all but reaches 1.0 OD when assay recommendations are followed. We recommend using a higher-sensitivity TMB substrate or extending TMB incubation time considerably.

The tested sample dilutions all had blank-subtracted OD less than 0.05. Spiked human protein was recoverable at 1:40 (60%).

Overall, we do not believe this kit is capable of detecting TAT complexes in plasma of rhesus macaque.
Username

Abcam user community

Verified customer

Submitted Jun 06 2016

Abreviews
Stored serum samples (-20) were used from rhesus macaque monkeys (NHP), 1:5 sample dilution with Abcam TAT ELISA kit used as per protocol to measure Thrombin-Anti-Thrombin complexes post-transplant, see attached image.
Colour development was faster (3-5m) than the c.20 mins anticipated in the protocol.
Username

Abcam user community

Verified customer

Submitted Nov 02 2015



All our kits are guaranteed for the first six months. But having said that, stored at the right conditions, the reagents could last up to a year.





TAT Complexes Standard: Reconstitute the 240 ng of human TAT Complexes Standard with 2 ml of MIX Diluent to generate a standard solution of 120 ng/ml. Allow the standard to sit for 10 minutes with gentle agitation prior to making d...

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Thank you for contacting us.

The catalogue number is ab108907

http://www.abcam.com/Thrombin-Antithrombin-Complex-TAT-Human-ELISA-Kit-ab108907.html

Please find attached the datasheet.

I hope this information is helpfu...

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Thank you for contacting us.

I am sorry you could not find this information in the protocol book. The range for this kit is stated on our datasheet and corresponds to 0.48 ng/ml - 120 ng/ml.

I hope this helps and if we can assist fu...

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The range for the strandard is from 1.48 to 120 ng/ml, as shown on pages 8 and 9 of the booklet: http://www.abcam.com/ps/products/108/ab108907/documents/ab108907%20Thrombin%20Antithrombin%20Complex%20Human%20ELISA%20Kit%20(Web)%20v2.pdf.

...

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Thank you for contacting Abcam.
Unfortunately ab108907 will not work well with mouse samples. We do have a few other kits that work on mouse that may be suitable though:
http://www.abcam.com/Antithrombin-III-Mouse-ELISA-Kit-ab108800.html
ht...

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Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

I am pleased to hear the kit has worked well for you. In answer to your questions:

1. Yes, as stated in the protocol ...

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Thank you for your patience.

I have heard back from the lab with the following information:

We have not yet tested the kit in whole blood. However, we tested bovine samples. The cross-reactivity is very low, around 2%.

T...

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1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"