Overview

  • Product nameHuman Thrombomodulin ELISA Kit (CD141)
    See all Thrombomodulin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 6 3.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 6 9.8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.31 ng/ml
  • Range
    0.625 ng/ml - 20 ng/ml
  • Recovery

    109 %

  • Assay time
    1h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thrombomodulin (CD141) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombomodulin (CD141) in serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for Thrombomodulin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Thrombomodulin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Thrombomodulin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Thrombomodulin present in the samples.

    This kit will recognize both endogenous and recombinant Human Thrombomodulin.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionThrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • Tissue specificityEndothelial cells are unique in synthesizing thrombomodulin.
  • Involvement in diseaseDefects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similaritiesContains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • Post-translational
    modifications
    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • AHUS 6
    • AHUS6
    • BDCA 3
    • BDCA3
    • CD 141
    • CD141
    • CD141 antigen
    • Fetomodulin
    • Thbd
    • THPH12
    • THRM
    • Thrombomodulin
    • TM
    • TRBM_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Thrombomodulin ELISA Kit (CD141) images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Thrombomodulin measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 2-16 fold.

Protocols

References for Human Thrombomodulin ELISA Kit (CD141) (ab46508)

This product has been referenced in:
  • Langdown J  et al. A hereditary bleeding disorder resulting from a premature stop codon in thrombomodulin (p.Cys537Stop). Blood 124:1951-6 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25049278) »
  • Poursafa P  et al. The relationship of air pollution and surrogate markers of endothelial dysfunction in a population-based sample of children. BMC Public Health 11:115 (2011). Read more (PubMed: 21332998) »

See all 3 Publications for this product

Product Wall

Simple & easy human thrombomodulin ELISA

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Followed the data sheet in detail to measure thrombomodulin in human serum.
Well made product and easy to understand.
Would recommend.
Username

Abcam user community

Verified customer

Submitted Jun 25 2014

Thanks for your enquiry.

My colleague is out of office and he has asked me to look after her customers during her absence.


The whole Thrombomodulin kit is stable if refrigerate (4°C) until at least for one year. However, w...

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Thank you very much for your understanding. The things are more clearer now. Lab has confirmed that the number K100624 (typos K10624) is indeed stands for standard vial.

They have also confirmed that the standard conc. should be between 11-16...

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Thank you for your inquiry.


A panel of 40 human sera and 40 plasma samples were tested for CD141 in a recent batch. See results below

Sample Matrix Number of samples evaluated Range (ng/ml) Mean (ng/ml) Standard deviation (ng...

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Thank you for your telephone enquiry earlier this afternoon. I can confirm that the concentration of the streptavidin - HRP in the Human Thrombomodulin ELISA Kit is 1.0mg/ml. I hope this is helpful. If you have any further questions, please do n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"