Human Thrombopoietin ELISA Kit(ab100656)
Human Thrombopoietin ELISA Kit
|Sample type||Average %||Range|
|Cell culture supernatant||97.82||84% - 105%|
|Plasma||93.44||80% - 103%|
Abcam’s Thrombopoietin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombopoietin in plasma and cell culture supernatants. Human Thrombopoietin concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human Thrombopoietin in normal serum with ab100656, only in serum spiked with Human Thrombopoietin.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|700X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human Thrombopoietin (lyophilized)||2 vials|
|Recombinant Human Thrombopoietin Standard||2 vials|
|Stop Solution||1 x 8ml|
|Thrombopoietin Microplate (12 x 8 wells)||1 unit|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100656 has not yet been referenced specifically in any publications.