Overview

  • Product name

    Human Thrombopoietin ELISA Kit


    See all Thrombopoietin kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    24.69 pg/ml - 6000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.82 84% - 105%
    Plasma 93.44 80% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thrombopoietin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombopoietin in plasma and cell culture supernatants. Human Thrombopoietin concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human Thrombopoietin in normal serum with ab100656, only in serum spiked with Human Thrombopoietin.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Relevance
    Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
  • Cellular localization
    Secreted
  • Alternative names
    • C mpl ligand
    • Megakaryocyte colony stimulating factor
    • Megakaryocyte growth and development factor
    • Megakaryocyte stimulating factor
    • MGC163194
    • MGDF
    • MKCSF
    • ML
    • MPL ligand
    • MPLLG
    • Myeloproliferative leukemia virus oncogene ligand
    • OTTHUMP00000210504
    • THPO
    • TPO
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Thrombopoietin ELISA Kit

images

  • Representative standard curve using ab100656

  • Representative standard curve using ab100656

Protocols

References for

Human Thrombopoietin ELISA Kit

(ab100656)

ab100656 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihren Anruf.

Die beiden monoklonalen Antikörper in diesem Kit sind gegen die Sequenz von ASXX-YY gerichtet, allerdings wurde das Epitop nicht weiter bestimmt. Trotzdem ist es sehr wahrscheinlich, dass das Kit mit Ihre...

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The developer of the kit has sent the following reply addressing your questions about the Thrombopoietin Human ELISA Kit. 1. The plasma samples are collected with the anti-coagulant ACD (acid citrate dextrose) instead of heparin or EDTA. Will that b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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