Overview

  • Product name
    Human Thrombospondin 1 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    6 ng/ml
  • Range
    6 ng/ml - 2000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91.67 79% - 113%
    Plasma 83.27 67% - 109%
    Cell culture media 86.97 73% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Thrombospondin 1 Human ELISA Kit (ab193716) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombospondin 1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human Thrombospondin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Thrombospondin 1 present in the samples. The wells are washed and biotinylated Human Thrombospondin 1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Thrombospondin 1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent D 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Biotinylated Human Thrombospondin detection antibody (lyophilized) 2 vials
    Human Thrombospondin Standards 2 vials
    Pre-coated Human Thrombospondin Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Function
    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.
  • Sequence similarities
    Belongs to the thrombospondin family.
    Contains 2 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Cellular localization
    Endoplasmic reticulum. Sarcoplasmic reticulum.
  • Information by UniProt
  • Alternative names
    • THBS
    • THBS 1
    • Thbs1
    • Thrombospondin-1
    • TSP
    • TSP 1
    • TSP1
    • TSP1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve using ab193716 Thrombospondin 1 Human ELISA Kit.

Protocols

References

ab193716 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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