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Abcam’s anti-Thyroid Peroxidase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroid peroxidase in Human serum or plasma (citrate).
A 96-well plate has been precoated with recombinant thyroid peroxidase to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroid peroxidase-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroid peroxidase sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover Foil||1 unit|
|IgG Sample Diluent||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|Thyroid peroxidase Anti-IgG HRP Conjugate||1 x 20ml|
|Thyroid peroxidase Coated Microplate (12 x 8 wells)||1 unit|
|Thyroid peroxidase Negative Control||1 x 2ml|
|Thyroid peroxidase Positive Control||1 x 2ml|
|Thyroid peroxidase Standard A - 0 U/mL||1 x 2ml|
|Thyroid peroxidase Standard B - 5 U/mL||1 x 2ml|
|Thyroid peroxidase Standard C - 10 U/mL||1 x 2ml|
|Thyroid peroxidase Standard D - 20 U/mL||1 x 2ml|
|Thyroid peroxidase Standard E - 80 U/mL||1 x 2ml|
|Thyroid peroxidase Standard F - 320 U/mL||1 x 2ml|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab178632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178632 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"