Human Thyroid Stimulating Hormone ELISA Kit (TSH) (ab100660)

Overview

  • Product nameHuman Thyroid Stimulating Hormone ELISA Kit (TSH)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    2.744 pg/ml - 2000 pg/ml
  • Recovery

    86 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82.76 70% - 90%
    Serum 97.89 75% - 120%
    Plasma 78.59 67% - 86%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TSH Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TSH in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
  • Cellular localizationSecreted
  • Alternative names
    • Anterior pituitary glycoprotein hormones common subunit alpha
    • CG ALPHA
    • CGA
    • CGA protein
    • Choriogonadotropin alpha chain
    • Chorionic gonadotrophin alpha subunit
    • Chorionic gonadotropin alpha polypeptide
    • Follicle stimulating hormone alpha chain
    • Follitropin alpha chain
    • FSH alpha
    • FSHA
    • Glycoprotein hormones alpha chain
    • Glycoprotein hormones alpha polypeptide
    • GPHa
    • GPHA1
    • HCG
    • LHA
    • LSH alpha
    • Luteinizing hormone alpha chain
    • Lutropin alpha chain
    • Thyroid stimulating hormone
    • Thyroid stimulating hormone alpha chain
    • Thyroid stimulating hormone beta
    • Thyroid stimulating hormone beta subunit
    • Thyroid stimulating hormone subunit beta
    • Thyroid Stimulation Hormone
    • Thyrotropin
    • Thyrotropin alfa
    • Thyrotropin alpha chain
    • Thyrotropin beta subunit
    • Thyrotropin subunit beta
    • TSH A
    • TSH alpha
    • TSH B
    • TSH beta
    • TSHA
    • TSHB
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Thyroid Stimulating Hormone ELISA Kit (TSH) images

  • Representative standard curve using ab100660

  • Representative standard curve using ab100660

Protocols

References for Human Thyroid Stimulating Hormone ELISA Kit (TSH) (ab100660)

ab100660 has not yet been referenced specifically in any publications.

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I am happy to help explain the vial contents for the ELH-TSH standard. Each vial is enough to run a duplicate standard curve. Given the concentration and volumes described in the manual...

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The catalog numbers ab108659 and ab108660 come from the same product line and are easy to compare. The TSH ELISA Kit ab108659 comes with standards in the range of 0-25 uIU/ml, while the TSH ELISA ab108660 is consi...

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