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Abcam’s Thyroid Stimulating Hormone in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroid Stimulating Hormone in Human serum.
A 96-well plate has been precoated with anti-Thyroid Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Thyroid Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Thyroid Stimulating Hormone HRP conjugate binds to the antibody-Thyroid Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Thyroid Stimulating Hormone in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Thyroid Stimulating Hormone Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Thyroid Stimulating Hormone HRP Conjugate||1 x 13ml|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
|TSH Standard 0 - 0 µU/mL (Lyophilized)||1 vial|
|TSH Standard 5 - 25 µU/mL (Lyophilized)||1 vial|
|TSH Standard 1 - 0.5 µU/mL (Lyophilized)||1 vial|
|TSH Standard 2 - 2 µU/mL (Lyophilized)||1 vial|
|TSH Standard 3 - 5 µU/mL (Lyophilized)||1 vial|
|TSH Standard 4 - 10 µU/mL (Lyophilized)||1 vial|
Our Abpromise guarantee covers the use of ab108659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative standard curve using ab108659
ab108659 has not yet been referenced specifically in any publications.