Human Thyroid Stimulating Hormone ELISA Kit (TSH) (ab108659)


  • Product nameHuman Thyroid Stimulating Hormone ELISA Kit (TSH)
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.05 U/ml
  • Range
    0.05 µlU/ml - 10 µlU/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thyroid Stimulating Hormone in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroid Stimulating Hormone in Human serum.

    A 96-well plate has been precoated with anti-Thyroid Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Thyroid Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Thyroid Stimulating Hormone HRP conjugate binds to the antibody-Thyroid Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Thyroid Stimulating Hormone in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate



Our Abpromise guarantee covers the use of ab108659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Thyroid Stimulating Hormone ELISA Kit (TSH) images

  • Representative standard curve using ab108659


References for Human Thyroid Stimulating Hormone ELISA Kit (TSH) (ab108659)

ab108659 has not yet been referenced specifically in any publications.

Product Wall

ab108659 does not detect TSH in naked mole-rat plasma

Inconclusive Excellent 5/5 (Ease of Use)
This kit was easy to use however, it did not detect TSH in naked mole-rat plasma. The standards worked perfectly, but my experimental samples failed.

Abcam user community

Verified customer

Submitted Jul 20 2015

I just wanted to follow up with you regarding this protein standard.

My colleague Caitlin mentioned she had been in contact with you and that you were interested in getting this protein as separate catalog number, essentially a similar protei...

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Thank you for contacting us.

The catalog numbers ab108659 and ab108660 come from the same product line and are easy to compare. The TSH ELISA Kit ab108659 comes with standards in the range of 0-25 uIU/ml, while the TSH ELISA ab108660 is consi...

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It looks like we may be able to providestandard from this same lot. I am waiting for some more information on the availability and will follow up with you as soon as possible.

In the meantime please let me know if you have any questions.

The standards for this kit are lot specific. If we have the lot you require in stock, we can provide it to separately.

Thanks in advance for the lot information on your kit. Please let me know if you have any further questions.

Thank you for contacting Abcam.

I have heard back from the lab and they have told me that the antibodies are not the same for the three kits. I apologize the information regarding these antibodies is not available as it is considered proprie...

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